The FTIRI method is mainly used to get information on the maturity of the mineral and on the organic component of the mineralized tissue. Both of these outcomes are greatly dependent on tissue age and are very sensitive to pharmaceutical interventions.
Several absorbance bands are determined for bone tissue for:
i) the estimation of the relative amount of mineral to organic matter; (ii) the stoichiometry of the apatite mineral for the characterization of the maturity of the mineral; (iii) the relative carbonate content of the hydroxyapatite – a second indicator for the maturity of the mineral; (iv) and the ratio of the non-reducible/reducible collagen cross-links in bone and thus the maturity of the collagen.
For details see Paschalis et al, Calcified Tissue International, 59:480-487, 1996; Paschalis et al, J of Bone and Mineral Research, 16(10), 1821-8, 2001.