
Methods
Spatial distribution of mineral crystallite maturity Spatial distribution of collagen cross-link ratio at the 6.3 mm spatial level Attenuated total reflectance FTIR spectroscopy coupled to a flow through chamber for real-time analysis of extracellular matrix in in situ cell cultures. Spatial distribution of mineral characteristics at the 0.6-1 mm spatial level Mineral crystallite and collagen fiber orientation Culture of primary cells and established cell lines. Techniques for studying gene expression including gene transfection and reporter gene assay. Analysis of epigenetic DNA methylation. Gene array analysis. Biochemical analysis of proteins including protein-DNA interaction. Cloning and recombinant protein techniques. Structural parameters, parameters static bone formation and resorption, dynamic bone formation. Pathohistological, diagnostic evaluatiof on of bone biopsies. 3-D fluorescence imaging of labelled bone tissue, cellular structures & cytoskeletal architecture. Imaging of resorption lacunae in in vitro assays. Bone mineral density distribution (BMDD) in a spatially resolved manner at the micrometer-range. Information of bone mineral crystallites characteristics in a spatially resolved manner at the nanometer range. Spatial distribution of elastic properties of bone composite. Read more about Nano-Indentation Spatially resolved acoustic properties of bone material at the micrometer-range. Architecture/structure of mineralized bone sample Clinical diagnosis Classification and characterization of patients with bone diseases
FTIRI (Fourier transform infrared imaging)
Read more about FTIRI (Fourier transform infrared imaging)ATR-FTIR (Attenuated total reflectance Fourier transform infrared)
RAMAN
Proteoglycans
Nanoporosity
Spatial distributuion of pyridinoline collagen cross-link content
Lipids
AGEs
Pyrophosphate
Read more about RAMANCellular & Molecular Biology
Light Microscopy/Histomorphometry
Read more about Light Microscopy/HistomorphometryConfocal Laser Scanning Microscopy
qBEI (quantitative backscattered electron imaging)
Read more about qBEI (quantitative backscattered electron imaging)EDX (energy dispersive X-ray micro-analysis)
Elemental composition of bone (sensitivity of quantification 0.1%)HR-BEI (high resolution backscatter electron imaging)
Visualization of bone matrix in nm-range (limit 4nm)Scanning SAXS (Small-angle X-ray scattering)
Read more about Scanning SAXS (Small-angle X-ray scattering)Nanoindentation
SASAM (Saarland scanning acoustic microscopy)
Micro-computed tomography (micro-CT) instrument shared with H. Redl
Conventional X-ray DXA, CT, MRT, Bone Scan